Construct: shRNA TRCN0000417887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032438.1-1461s21c1
DNA Barcode:
GAACATAGAAGAACCCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
L3MBTL3 (84456)
Gene Description:
L3MBTL histone methyl-lysine binding protein 3
Transcript:
RefSeq NM_032438.1 (NON-CURRENT)
Match location:
Position 1461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACATAGAAGAACCCTTATT
Hairpin Sequence:
5'-CCGG-GAACATAGAAGAACCCTTATT-CTCGAG-AATAAGGGTTCTTCTATGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACATAGAAGAACCCTTATTCTCGAGAATAAGGGTTCTTCTATGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACATAGAAGAACCCTTATTCTCGAGAATAAGGGTTCTTCTATGTTC-3'

Other clones with same target sequence:

(none)