Construct: shRNA TRCN0000417999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152396.2-1114s21c1
DNA Barcode:
CCTTTCATGAGGTTGGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
METTL6 (131965)
Gene Description:
methyltransferase like 6
Transcript:
RefSeq NM_152396.2 (NON-CURRENT)
Match location:
Position 1114 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCATGAGGTTGGCATTT
Hairpin Sequence:
5'-CCGG-CCTTTCATGAGGTTGGCATTT-CTCGAG-AAATGCCAACCTCATGAAAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCATGAGGTTGGCATTTCTCGAGAAATGCCAACCTCATGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCATGAGGTTGGCATTTCTCGAGAAATGCCAACCTCATGAAAGG-3'

Other clones with same target sequence:

(none)