Construct: shRNA TRCN0000418002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153138.3-1802s21c1
DNA Barcode:
TCCTGAGCTATTGCTTGTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wipf1 (215280)
Gene Description:
WAS/WASL interacting protein family, member 1
Transcript:
RefSeq NM_153138.3 (NON-CURRENT)
Match location:
Position 1802 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTGAGCTATTGCTTGTATC
Hairpin Sequence:
5'-CCGG-TCCTGAGCTATTGCTTGTATC-CTCGAG-GATACAAGCAATAGCTCAGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTGAGCTATTGCTTGTATCCTCGAGGATACAAGCAATAGCTCAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTGAGCTATTGCTTGTATCCTCGAGGATACAAGCAATAGCTCAGGA-3'

Other clones with same target sequence:

(none)