Construct: shRNA TRCN0000418022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018751.2-953s21c1
DNA Barcode:
GAGACTGGAAGAACTACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sult1c1 (20888)
Gene Description:
sulfotransferase family, cytosolic, 1C, member 1
Transcript:
RefSeq NM_018751.2 (CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTGGAAGAACTACTTTA
Hairpin Sequence:
5'-CCGG-GAGACTGGAAGAACTACTTTA-CTCGAG-TAAAGTAGTTCTTCCAGTCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTGGAAGAACTACTTTACTCGAGTAAAGTAGTTCTTCCAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTGGAAGAACTACTTTACTCGAGTAAAGTAGTTCTTCCAGTCTC-3'

Other clones with same target sequence:

(none)