Construct: shRNA TRCN0000418064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173075.4-1025s21c1
DNA Barcode:
CTGAGAAGTTAGAGGGTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APBB2 (323)
Gene Description:
amyloid beta precursor protein binding family B member 2
Transcript:
RefSeq NM_173075.4 (NON-CURRENT)
Match location:
Position 1025 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGAAGTTAGAGGGTAAAG
Hairpin Sequence:
5'-CCGG-CTGAGAAGTTAGAGGGTAAAG-CTCGAG-CTTTACCCTCTAACTTCTCAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGAAGTTAGAGGGTAAAGCTCGAGCTTTACCCTCTAACTTCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGAAGTTAGAGGGTAAAGCTCGAGCTTTACCCTCTAACTTCTCAG-3'

Other clones with same target sequence:

(none)