Construct: shRNA TRCN0000418084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015947.2-216s21c1
DNA Barcode:
AGAATTGATGGCCGGAATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASCC1 (51008)
Gene Description:
activating signal cointegrator 1 complex subunit 1
Transcript:
RefSeq NM_015947.2 (NON-CURRENT)
Match location:
Position 216 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATTGATGGCCGGAATTAC
Hairpin Sequence:
5'-CCGG-AGAATTGATGGCCGGAATTAC-CTCGAG-GTAATTCCGGCCATCAATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATTGATGGCCGGAATTACCTCGAGGTAATTCCGGCCATCAATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATTGATGGCCGGAATTACCTCGAGGTAATTCCGGCCATCAATTCT-3'

Other clones with same target sequence:

(none)