Construct: shRNA TRCN0000418139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018231.1-1029s21c1
DNA Barcode:
GTACGTCACAGCCATCGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC38A7 (55238)
Gene Description:
solute carrier family 38 member 7
Transcript:
RefSeq NM_018231.1 (NON-CURRENT)
Match location:
Position 1029 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGTCACAGCCATCGTTAT
Hairpin Sequence:
5'-CCGG-GTACGTCACAGCCATCGTTAT-CTCGAG-ATAACGATGGCTGTGACGTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGTCACAGCCATCGTTATCTCGAGATAACGATGGCTGTGACGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGTCACAGCCATCGTTATCTCGAGATAACGATGGCTGTGACGTAC-3'

Other clones with same target sequence:

(none)