Construct: shRNA TRCN0000418162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019014.4-2302s21c1
DNA Barcode:
CGCAGATTCTCTTCGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR1B (84172)
Gene Description:
RNA polymerase I subunit B
Transcript:
RefSeq NM_019014.4 (NON-CURRENT)
Match location:
Position 2302 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGATTCTCTTCGTCATTT
Hairpin Sequence:
5'-CCGG-CGCAGATTCTCTTCGTCATTT-CTCGAG-AAATGACGAAGAGAATCTGCG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGATTCTCTTCGTCATTTCTCGAGAAATGACGAAGAGAATCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGATTCTCTTCGTCATTTCTCGAGAAATGACGAAGAGAATCTGCG-3'

Other clones with same target sequence:

(none)