Construct: shRNA TRCN0000418287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014751.4-3133s21c1
DNA Barcode:
GGGAACCACGAAGTAGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTSS1 (9788)
Gene Description:
MTSS I-BAR domain containing 1
Transcript:
RefSeq NM_014751.4 (NON-CURRENT)
Match location:
Position 3133 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAACCACGAAGTAGTTAAT
Hairpin Sequence:
5'-CCGG-GGGAACCACGAAGTAGTTAAT-CTCGAG-ATTAACTACTTCGTGGTTCCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAACCACGAAGTAGTTAATCTCGAGATTAACTACTTCGTGGTTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAACCACGAAGTAGTTAATCTCGAGATTAACTACTTCGTGGTTCCC-3'

Other clones with same target sequence:

(none)