Construct: shRNA TRCN0000418320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021938.3-1718s21c1
DNA Barcode:
TGGTGTTCCTGTTACGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CELF5 (60680)
Gene Description:
CUGBP Elav-like family member 5
Transcript:
RefSeq NM_021938.3 (NON-CURRENT)
Match location:
Position 1718 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGTTCCTGTTACGTGTTT
Hairpin Sequence:
5'-CCGG-TGGTGTTCCTGTTACGTGTTT-CTCGAG-AAACACGTAACAGGAACACCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGTTCCTGTTACGTGTTTCTCGAGAAACACGTAACAGGAACACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGTTCCTGTTACGTGTTTCTCGAGAAACACGTAACAGGAACACCA-3'

Other clones with same target sequence:

(none)