Construct: shRNA TRCN0000418322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138403.4-528s21c1
DNA Barcode:
GCCGCATCAATGTCAAGAACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL10 (93408)
Gene Description:
myosin light chain 10
Transcript:
RefSeq NM_138403.4 (NON-CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCATCAATGTCAAGAACG
Hairpin Sequence:
5'-CCGG-GCCGCATCAATGTCAAGAACG-CTCGAG-CGTTCTTGACATTGATGCGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCATCAATGTCAAGAACGCTCGAGCGTTCTTGACATTGATGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCATCAATGTCAAGAACGCTCGAGCGTTCTTGACATTGATGCGGC-3'

Other clones with same target sequence:

(none)