Construct: shRNA TRCN0000418335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014918.4-1429s21c1
DNA Barcode:
ACATGCTGAGCCGCAAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHSY1 (22856)
Gene Description:
chondroitin sulfate synthase 1
Transcript:
RefSeq NM_014918.4 (NON-CURRENT)
Match location:
Position 1429 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATGCTGAGCCGCAAGATAT
Hairpin Sequence:
5'-CCGG-ACATGCTGAGCCGCAAGATAT-CTCGAG-ATATCTTGCGGCTCAGCATGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATGCTGAGCCGCAAGATATCTCGAGATATCTTGCGGCTCAGCATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATGCTGAGCCGCAAGATATCTCGAGATATCTTGCGGCTCAGCATGT-3'

Other clones with same target sequence:

(none)