Construct: shRNA TRCN0000418409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000547.5-2958s21c1
DNA Barcode:
CAAACACAGGCAAATCCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPO (7173)
Gene Description:
thyroid peroxidase
Transcript:
RefSeq NM_000547.5 (CURRENT)
Match location:
Position 2958 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACACAGGCAAATCCGAAA
Hairpin Sequence:
5'-CCGG-CAAACACAGGCAAATCCGAAA-CTCGAG-TTTCGGATTTGCCTGTGTTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACACAGGCAAATCCGAAACTCGAGTTTCGGATTTGCCTGTGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACACAGGCAAATCCGAAACTCGAGTTTCGGATTTGCCTGTGTTTG-3'

Other clones with same target sequence:

(none)