Construct: shRNA TRCN0000418473

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024726.3-230s21c1
DNA Barcode:
CCTCGGTGCTTTACTCGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQCA1 (79781)
Gene Description:
IQ motif containing with AAA domain 1
Transcript:
RefSeq NM_024726.3 (NON-CURRENT)
Match location:
Position 230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418473)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGGTGCTTTACTCGATAA
Hairpin Sequence:
5'-CCCG-CCTCGGTGCTTTACTCGATAA-CTCGAG-TTATCGAGTAAAGCACCGAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGGTGCTTTACTCGATAACTCGAGTTATCGAGTAAAGCACCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGGTGCTTTACTCGATAACTCGAGTTATCGAGTAAAGCACCGAGG-3'

Other clones with same target sequence:

(none)