Construct: shRNA TRCN0000418635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005509.4-6757s21c1
DNA Barcode:
AGTAGTTGCCAATCCATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DMXL1 (1657)
Gene Description:
Dmx like 1
Transcript:
RefSeq NM_005509.4 (NON-CURRENT)
Match location:
Position 6757 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAGTTGCCAATCCATTATT
Hairpin Sequence:
5'-CCGG-AGTAGTTGCCAATCCATTATT-CTCGAG-AATAATGGATTGGCAACTACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAGTTGCCAATCCATTATTCTCGAGAATAATGGATTGGCAACTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAGTTGCCAATCCATTATTCTCGAGAATAATGGATTGGCAACTACT-3'

Other clones with same target sequence:

  1. TRCN0000242011