Construct: shRNA TRCN0000418688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003053.3-1007s21c1
DNA Barcode:
GAAGTGTAATGTACGAGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC18A1 (6570)
Gene Description:
solute carrier family 18 member A1
Transcript:
RefSeq NM_003053.3 (NON-CURRENT)
Match location:
Position 1007 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGTAATGTACGAGTTTG
Hairpin Sequence:
5'-CCGG-GAAGTGTAATGTACGAGTTTG-CTCGAG-CAAACTCGTACATTACACTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGTAATGTACGAGTTTGCTCGAGCAAACTCGTACATTACACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGTAATGTACGAGTTTGCTCGAGCAAACTCGTACATTACACTTC-3'

Other clones with same target sequence:

(none)