Construct: shRNA TRCN0000418715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153252.3-5849s21c1
DNA Barcode:
TCAAATAAGCCTTACTCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRWD3 (254065)
Gene Description:
bromodomain and WD repeat domain containing 3
Transcript:
RefSeq NM_153252.3 (NON-CURRENT)
Match location:
Position 5849 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAATAAGCCTTACTCATTA
Hairpin Sequence:
5'-CCGG-TCAAATAAGCCTTACTCATTA-CTCGAG-TAATGAGTAAGGCTTATTTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAATAAGCCTTACTCATTACTCGAGTAATGAGTAAGGCTTATTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAATAAGCCTTACTCATTACTCGAGTAATGAGTAAGGCTTATTTGA-3'

Other clones with same target sequence:

(none)