Construct: shRNA TRCN0000418721
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_003986.2-543s21c1
- DNA Barcode:
- GATGTGAACATTGGAATTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BBOX1 (8424)
- Gene Description:
- gamma-butyrobetaine hydroxylase 1
- Transcript:
- RefSeq NM_003986.2 (NON-CURRENT)
- Match location:
- Position 543 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATGTGAACATTGGAATTAAA
- Hairpin Sequence:
-
5'-CCGG-GATGTGAACATTGGAATTAAA-CTCGAG-TTTAATTCCAATGTTCACATC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATGTGAACATTGGAATTAAACTCGAGTTTAATTCCAATGTTCACATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATGTGAACATTGGAATTAAACTCGAGTTTAATTCCAATGTTCACATC-3'
Other clones with same target sequence:
(none)
