Construct: shRNA TRCN0000418744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015980.3-824s21c1
DNA Barcode:
GAGTTGTCTGAACCGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSG2 (51617)
Gene Description:
neuronal vesicle trafficking associated 2
Transcript:
RefSeq NM_015980.3 (NON-CURRENT)
Match location:
Position 824 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTGTCTGAACCGATATTT
Hairpin Sequence:
5'-CCGG-GAGTTGTCTGAACCGATATTT-CTCGAG-AAATATCGGTTCAGACAACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTGTCTGAACCGATATTTCTCGAGAAATATCGGTTCAGACAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTGTCTGAACCGATATTTCTCGAGAAATATCGGTTCAGACAACTC-3'

Other clones with same target sequence:

(none)