Construct: shRNA TRCN0000418754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003867.2-707s21c1
DNA Barcode:
CCTTTCCCTTCTTAATCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGF17 (8822)
Gene Description:
fibroblast growth factor 17
Transcript:
RefSeq NM_003867.2 (NON-CURRENT)
Match location:
Position 707 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCCCTTCTTAATCCAAG
Hairpin Sequence:
5'-CCGG-CCTTTCCCTTCTTAATCCAAG-CTCGAG-CTTGGATTAAGAAGGGAAAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCCCTTCTTAATCCAAGCTCGAGCTTGGATTAAGAAGGGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCCCTTCTTAATCCAAGCTCGAGCTTGGATTAAGAAGGGAAAGG-3'

Other clones with same target sequence:

(none)