Construct: shRNA TRCN0000418756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145942.4-402s21c1
DNA Barcode:
CAGAGACAATCATCGACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgcs1 (208715)
Gene Description:
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
Transcript:
RefSeq NM_145942.4 (NON-CURRENT)
Match location:
Position 402 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGACAATCATCGACAAAT
Hairpin Sequence:
5'-CCGG-CAGAGACAATCATCGACAAAT-CTCGAG-ATTTGTCGATGATTGTCTCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGACAATCATCGACAAATCTCGAGATTTGTCGATGATTGTCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGACAATCATCGACAAATCTCGAGATTTGTCGATGATTGTCTCTG-3'

Other clones with same target sequence:

(none)