Construct: shRNA TRCN0000418811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015382.2-7588s21c1
DNA Barcode:
GCTATCAAGAGGCGCCAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HECTD1 (25831)
Gene Description:
HECT domain E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_015382.2 (NON-CURRENT)
Match location:
Position 7588 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCAAGAGGCGCCAAATT
Hairpin Sequence:
5'-CCGG-GCTATCAAGAGGCGCCAAATT-CTCGAG-AATTTGGCGCCTCTTGATAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCAAGAGGCGCCAAATTCTCGAGAATTTGGCGCCTCTTGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCAAGAGGCGCCAAATTCTCGAGAATTTGGCGCCTCTTGATAGC-3'

Other clones with same target sequence:

(none)