Construct: shRNA TRCN0000418816

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021007.2-1132s21c1
DNA Barcode:
TAAGCGTGTTTGCGCTAATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN2A (6326)
Gene Description:
sodium voltage-gated channel alpha subunit 2
Transcript:
RefSeq NM_021007.2 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418816)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGCGTGTTTGCGCTAATAG
Hairpin Sequence:
5'-CCGG-TAAGCGTGTTTGCGCTAATAG-CTCGAG-CTATTAGCGCAAACACGCTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGCGTGTTTGCGCTAATAGCTCGAGCTATTAGCGCAAACACGCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGCGTGTTTGCGCTAATAGCTCGAGCTATTAGCGCAAACACGCTTA-3'

Other clones with same target sequence:

(none)