Construct: shRNA TRCN0000418847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021190.2-1311s21c1
DNA Barcode:
GTTTACCCTCTTCGGTGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTBP2 (58155)
Gene Description:
polypyrimidine tract binding protein 2
Transcript:
RefSeq NM_021190.2 (NON-CURRENT)
Match location:
Position 1311 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTACCCTCTTCGGTGTTTA
Hairpin Sequence:
5'-CCGG-GTTTACCCTCTTCGGTGTTTA-CTCGAG-TAAACACCGAAGAGGGTAAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTACCCTCTTCGGTGTTTACTCGAGTAAACACCGAAGAGGGTAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTACCCTCTTCGGTGTTTACTCGAGTAAACACCGAAGAGGGTAAAC-3'

Other clones with same target sequence:

(none)