Construct: shRNA TRCN0000418894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015156.2-1921s21c1
DNA Barcode:
ACAACCACCTAAGTGATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RCOR1 (23186)
Gene Description:
REST corepressor 1
Transcript:
RefSeq NM_015156.2 (NON-CURRENT)
Match location:
Position 1921 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACCACCTAAGTGATAATA
Hairpin Sequence:
5'-CCGG-ACAACCACCTAAGTGATAATA-CTCGAG-TATTATCACTTAGGTGGTTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACCACCTAAGTGATAATACTCGAGTATTATCACTTAGGTGGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACCACCTAAGTGATAATACTCGAGTATTATCACTTAGGTGGTTGT-3'

Other clones with same target sequence:

(none)