Construct: shRNA TRCN0000418920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014901.4-2209s21c1
DNA Barcode:
GAAGGTCTCATGCTCTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF44 (22838)
Gene Description:
ring finger protein 44
Transcript:
RefSeq NM_014901.4 (NON-CURRENT)
Match location:
Position 2209 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTCTCATGCTCTGAAAT
Hairpin Sequence:
5'-CCGG-GAAGGTCTCATGCTCTGAAAT-CTCGAG-ATTTCAGAGCATGAGACCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTCTCATGCTCTGAAATCTCGAGATTTCAGAGCATGAGACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTCTCATGCTCTGAAATCTCGAGATTTCAGAGCATGAGACCTTC-3'

Other clones with same target sequence:

(none)