Construct: shRNA TRCN0000418944

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003724.2-2391s21c1
DNA Barcode:
TGCCCGAGGTTCCTGTCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JRK (8629)
Gene Description:
Jrk helix-turn-helix protein
Transcript:
RefSeq NM_003724.2 (NON-CURRENT)
Match location:
Position 2391 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418944)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCCGAGGTTCCTGTCAATA
Hairpin Sequence:
5'-CCGG-TGCCCGAGGTTCCTGTCAATA-CTCGAG-TATTGACAGGAACCTCGGGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCCGAGGTTCCTGTCAATACTCGAGTATTGACAGGAACCTCGGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCCGAGGTTCCTGTCAATACTCGAGTATTGACAGGAACCTCGGGCA-3'

Other clones with same target sequence:

(none)