Construct: shRNA TRCN0000418951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144583.3-1527s21c1
DNA Barcode:
TTCATGTCTTAACGGCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V1C2 (245973)
Gene Description:
ATPase H+ transporting V1 subunit C2
Transcript:
RefSeq NM_144583.3 (NON-CURRENT)
Match location:
Position 1527 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATGTCTTAACGGCTATTT
Hairpin Sequence:
5'-CCGG-TTCATGTCTTAACGGCTATTT-CTCGAG-AAATAGCCGTTAAGACATGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATGTCTTAACGGCTATTTCTCGAGAAATAGCCGTTAAGACATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATGTCTTAACGGCTATTTCTCGAGAAATAGCCGTTAAGACATGAA-3'

Other clones with same target sequence:

(none)