Construct: shRNA TRCN0000418954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033394.2-935s21c1
DNA Barcode:
AGCAAGGACAGTGGAATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TANC1 (85461)
Gene Description:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Transcript:
RefSeq NM_033394.2 (NON-CURRENT)
Match location:
Position 935 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGGACAGTGGAATTATA
Hairpin Sequence:
5'-CCGG-AGCAAGGACAGTGGAATTATA-CTCGAG-TATAATTCCACTGTCCTTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGGACAGTGGAATTATACTCGAGTATAATTCCACTGTCCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGGACAGTGGAATTATACTCGAGTATAATTCCACTGTCCTTGCT-3'

Other clones with same target sequence:

(none)