Construct: shRNA TRCN0000418970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139057.2-3512s21c1
DNA Barcode:
CATTAGTGCTTCCGTACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMTS17 (170691)
Gene Description:
ADAM metallopeptidase with thrombospondin type 1 motif 17
Transcript:
RefSeq NM_139057.2 (NON-CURRENT)
Match location:
Position 3512 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000418970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTAGTGCTTCCGTACTTAA
Hairpin Sequence:
5'-CCGG-CATTAGTGCTTCCGTACTTAA-CTCGAG-TTAAGTACGGAAGCACTAATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTAGTGCTTCCGTACTTAACTCGAGTTAAGTACGGAAGCACTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTAGTGCTTCCGTACTTAACTCGAGTTAAGTACGGAAGCACTAATG-3'

Other clones with same target sequence:

(none)