Construct: shRNA TRCN0000419032

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152434.2-1810s21c1
DNA Barcode:
GATGATAATCTAAGCCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CWF19L2 (143884)
Gene Description:
CWF19 like cell cycle control factor 2
Transcript:
RefSeq NM_152434.2 (CURRENT)
Match location:
Position 1810 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419032)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATAATCTAAGCCTAAAT
Hairpin Sequence:
5'-CCGG-GATGATAATCTAAGCCTAAAT-CTCGAG-ATTTAGGCTTAGATTATCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATAATCTAAGCCTAAATCTCGAGATTTAGGCTTAGATTATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATAATCTAAGCCTAAATCTCGAGATTTAGGCTTAGATTATCATC-3'

Other clones with same target sequence:

(none)