Construct: shRNA TRCN0000419044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005603.3-1331s21c1
DNA Barcode:
AGCTCGGGCAGATCCATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP8B1 (5205)
Gene Description:
ATPase phospholipid transporting 8B1
Transcript:
RefSeq NM_005603.3 (NON-CURRENT)
Match location:
Position 1331 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTCGGGCAGATCCATTATA
Hairpin Sequence:
5'-CCGG-AGCTCGGGCAGATCCATTATA-CTCGAG-TATAATGGATCTGCCCGAGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTCGGGCAGATCCATTATACTCGAGTATAATGGATCTGCCCGAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTCGGGCAGATCCATTATACTCGAGTATAATGGATCTGCCCGAGCT-3'

Other clones with same target sequence:

(none)