Construct: shRNA TRCN0000419060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014456.3-653s21c1
DNA Barcode:
ATGTGAAAGATCCTAACTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDCD4 (27250)
Gene Description:
programmed cell death 4
Transcript:
RefSeq NM_014456.3 (NON-CURRENT)
Match location:
Position 653 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTGAAAGATCCTAACTATG
Hairpin Sequence:
5'-CCGG-ATGTGAAAGATCCTAACTATG-CTCGAG-CATAGTTAGGATCTTTCACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTGAAAGATCCTAACTATGCTCGAGCATAGTTAGGATCTTTCACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTGAAAGATCCTAACTATGCTCGAGCATAGTTAGGATCTTTCACAT-3'

Other clones with same target sequence:

(none)