Construct: shRNA TRCN0000419266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057177.5-2056s21c1
DNA Barcode:
GTAACCACCTCTAGGCGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARD3B (117583)
Gene Description:
par-3 family cell polarity regulator beta
Transcript:
RefSeq NM_057177.5 (NON-CURRENT)
Match location:
Position 2056 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAACCACCTCTAGGCGAAAT
Hairpin Sequence:
5'-CCGG-GTAACCACCTCTAGGCGAAAT-CTCGAG-ATTTCGCCTAGAGGTGGTTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAACCACCTCTAGGCGAAATCTCGAGATTTCGCCTAGAGGTGGTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAACCACCTCTAGGCGAAATCTCGAGATTTCGCCTAGAGGTGGTTAC-3'

Other clones with same target sequence:

(none)