Construct: shRNA TRCN0000419350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001939.2-3617s21c1
DNA Barcode:
GAAAGAGAGAAGCCTAATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DRP2 (1821)
Gene Description:
dystrophin related protein 2
Transcript:
RefSeq NM_001939.2 (CURRENT)
Match location:
Position 3617 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGAGAGAAGCCTAATTAC
Hairpin Sequence:
5'-CCGG-GAAAGAGAGAAGCCTAATTAC-CTCGAG-GTAATTAGGCTTCTCTCTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGAGAGAAGCCTAATTACCTCGAGGTAATTAGGCTTCTCTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGAGAGAAGCCTAATTACCTCGAGGTAATTAGGCTTCTCTCTTTC-3'

Other clones with same target sequence:

(none)