Construct: shRNA TRCN0000419370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173464.3-2454s21c1
DNA Barcode:
GTTCATGAATACATCGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
L3MBTL4 (91133)
Gene Description:
L3MBTL histone methyl-lysine binding protein 4
Transcript:
RefSeq NM_173464.3 (CURRENT)
Match location:
Position 2454 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCATGAATACATCGTTAAT
Hairpin Sequence:
5'-CCGG-GTTCATGAATACATCGTTAAT-CTCGAG-ATTAACGATGTATTCATGAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCATGAATACATCGTTAATCTCGAGATTAACGATGTATTCATGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCATGAATACATCGTTAATCTCGAGATTAACGATGTATTCATGAAC-3'

Other clones with same target sequence:

(none)