Construct: shRNA TRCN0000419387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018367.5-497s21c1
DNA Barcode:
AGTAACCACAGTTTACCTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACER3 (55331)
Gene Description:
alkaline ceramidase 3
Transcript:
RefSeq NM_018367.5 (NON-CURRENT)
Match location:
Position 497 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAACCACAGTTTACCTTAA
Hairpin Sequence:
5'-CCGG-AGTAACCACAGTTTACCTTAA-CTCGAG-TTAAGGTAAACTGTGGTTACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAACCACAGTTTACCTTAACTCGAGTTAAGGTAAACTGTGGTTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAACCACAGTTTACCTTAACTCGAGTTAAGGTAAACTGTGGTTACT-3'

Other clones with same target sequence:

(none)