Construct: shRNA TRCN0000419412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020981.2-1085s21c1
DNA Barcode:
CCGATGTAGCTGAACTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B3GALT1 (8708)
Gene Description:
beta-1,3-galactosyltransferase 1
Transcript:
RefSeq NM_020981.2 (NON-CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATGTAGCTGAACTCATTT
Hairpin Sequence:
5'-CCGG-CCGATGTAGCTGAACTCATTT-CTCGAG-AAATGAGTTCAGCTACATCGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATGTAGCTGAACTCATTTCTCGAGAAATGAGTTCAGCTACATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATGTAGCTGAACTCATTTCTCGAGAAATGAGTTCAGCTACATCGG-3'

Other clones with same target sequence:

(none)