Construct: shRNA TRCN0000419472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004196.2-1235s21c1
DNA Barcode:
GTCTGTTGTAGGACTTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD200 (4345)
Gene Description:
CD200 molecule
Transcript:
RefSeq NM_001004196.2 (NON-CURRENT)
Match location:
Position 1235 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGTTGTAGGACTTGATTT
Hairpin Sequence:
5'-CCGG-GTCTGTTGTAGGACTTGATTT-CTCGAG-AAATCAAGTCCTACAACAGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGTTGTAGGACTTGATTTCTCGAGAAATCAAGTCCTACAACAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGTTGTAGGACTTGATTTCTCGAGAAATCAAGTCCTACAACAGAC-3'

Other clones with same target sequence:

(none)