Construct: shRNA TRCN0000419497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032656.2-3716s21c1
DNA Barcode:
ATGAGAGCTCGATCCCGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX37 (57647)
Gene Description:
DEAH-box helicase 37
Transcript:
RefSeq NM_032656.2 (NON-CURRENT)
Match location:
Position 3716 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGAGCTCGATCCCGTATG
Hairpin Sequence:
5'-CCGG-ATGAGAGCTCGATCCCGTATG-CTCGAG-CATACGGGATCGAGCTCTCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGAGCTCGATCCCGTATGCTCGAGCATACGGGATCGAGCTCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGAGCTCGATCCCGTATGCTCGAGCATACGGGATCGAGCTCTCAT-3'

Other clones with same target sequence:

(none)