Construct: shRNA TRCN0000419523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020153.3-776s21c1
DNA Barcode:
CTATGAGCATTTGCCAGTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFT46 (56912)
Gene Description:
intraflagellar transport 46
Transcript:
RefSeq NM_020153.3 (CURRENT)
Match location:
Position 776 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGAGCATTTGCCAGTTTC
Hairpin Sequence:
5'-CCGG-CTATGAGCATTTGCCAGTTTC-CTCGAG-GAAACTGGCAAATGCTCATAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGAGCATTTGCCAGTTTCCTCGAGGAAACTGGCAAATGCTCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGAGCATTTGCCAGTTTCCTCGAGGAAACTGGCAAATGCTCATAG-3'

Other clones with same target sequence:

(none)