Construct: shRNA TRCN0000419524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000130.4-861s21c1
DNA Barcode:
ATGGGACAATGCCAGATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
F5 (2153)
Gene Description:
coagulation factor V
Transcript:
RefSeq NM_000130.4 (NON-CURRENT)
Match location:
Position 861 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGGACAATGCCAGATATAA
Hairpin Sequence:
5'-CCGG-ATGGGACAATGCCAGATATAA-CTCGAG-TTATATCTGGCATTGTCCCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGGACAATGCCAGATATAACTCGAGTTATATCTGGCATTGTCCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGGACAATGCCAGATATAACTCGAGTTATATCTGGCATTGTCCCAT-3'

Other clones with same target sequence:

(none)