Construct: shRNA TRCN0000419548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007139.2-1525s21c1
DNA Barcode:
CACTGAAGGGAAATCCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF92 (168374)
Gene Description:
zinc finger protein 92
Transcript:
RefSeq NM_007139.2 (NON-CURRENT)
Match location:
Position 1525 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGAAGGGAAATCCTACAA
Hairpin Sequence:
5'-CCGG-CACTGAAGGGAAATCCTACAA-CTCGAG-TTGTAGGATTTCCCTTCAGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGAAGGGAAATCCTACAACTCGAGTTGTAGGATTTCCCTTCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGAAGGGAAATCCTACAACTCGAGTTGTAGGATTTCCCTTCAGTG-3'

Other clones with same target sequence:

(none)