Construct: shRNA TRCN0000419570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005923.3-4786s21c1
DNA Barcode:
GCTATTGCACTTCAGCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K5 (4217)
Gene Description:
mitogen-activated protein kinase kinase kinase 5
Transcript:
RefSeq NM_005923.3 (NON-CURRENT)
Match location:
Position 4786 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTGCACTTCAGCCAAAT
Hairpin Sequence:
5'-CCGG-GCTATTGCACTTCAGCCAAAT-CTCGAG-ATTTGGCTGAAGTGCAATAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTGCACTTCAGCCAAATCTCGAGATTTGGCTGAAGTGCAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTGCACTTCAGCCAAATCTCGAGATTTGGCTGAAGTGCAATAGC-3'

Other clones with same target sequence:

(none)