Construct: shRNA TRCN0000419578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016020.2-461s21c1
DNA Barcode:
CCTCCAAATGTACATATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TFB1M (51106)
Gene Description:
transcription factor B1, mitochondrial
Transcript:
RefSeq NM_016020.2 (NON-CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAAATGTACATATTATT
Hairpin Sequence:
5'-CCGG-CCTCCAAATGTACATATTATT-CTCGAG-AATAATATGTACATTTGGAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAAATGTACATATTATTCTCGAGAATAATATGTACATTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAAATGTACATATTATTCTCGAGAATAATATGTACATTTGGAGG-3'

Other clones with same target sequence:

(none)