Construct: shRNA TRCN0000419583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172070.3-5450s21c1
DNA Barcode:
TGATCAATGCATCGGTAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBR3 (130507)
Gene Description:
ubiquitin protein ligase E3 component n-recognin 3
Transcript:
RefSeq NM_172070.3 (NON-CURRENT)
Match location:
Position 5450 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCAATGCATCGGTAATTA
Hairpin Sequence:
5'-CCGA-TGATCAATGCATCGGTAATTA-CTCGAG-TAATTACCGATGCATTGATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCAATGCATCGGTAATTACTCGAGTAATTACCGATGCATTGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCAATGCATCGGTAATTACTCGAGTAATTACCGATGCATTGATCA-3'

Other clones with same target sequence:

(none)