Construct: shRNA TRCN0000419640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027642.1-888s21c1
DNA Barcode:
CAAGAGGGAAATTGCATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf6 (70998)
Gene Description:
PHD finger protein 6
Transcript:
RefSeq NM_027642.1 (NON-CURRENT)
Match location:
Position 888 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGGGAAATTGCATATAT
Hairpin Sequence:
5'-CCGG-CAAGAGGGAAATTGCATATAT-CTCGAG-ATATATGCAATTTCCCTCTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGGGAAATTGCATATATCTCGAGATATATGCAATTTCCCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGGGAAATTGCATATATCTCGAGATATATGCAATTTCCCTCTTG-3'

Other clones with same target sequence:

(none)