Construct: shRNA TRCN0000419660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011552.3-3188s21c1
DNA Barcode:
GTAATTCACATTGCCGATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9A4 (389015)
Gene Description:
solute carrier family 9 member A4
Transcript:
RefSeq NM_001011552.3 (NON-CURRENT)
Match location:
Position 3188 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAATTCACATTGCCGATATG
Hairpin Sequence:
5'-CCGG-GTAATTCACATTGCCGATATG-CTCGAG-CATATCGGCAATGTGAATTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAATTCACATTGCCGATATGCTCGAGCATATCGGCAATGTGAATTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAATTCACATTGCCGATATGCTCGAGCATATCGGCAATGTGAATTAC-3'

Other clones with same target sequence:

(none)