Construct: shRNA TRCN0000419664

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178815.3-361s21c1
DNA Barcode:
TCTTAATGAATGAAGTGGTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARL5B (221079)
Gene Description:
ADP ribosylation factor like GTPase 5B
Transcript:
RefSeq NM_178815.3 (NON-CURRENT)
Match location:
Position 361 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419664)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTAATGAATGAAGTGGTTC
Hairpin Sequence:
5'-CCGG-TCTTAATGAATGAAGTGGTTC-CTCGAG-GAACCACTTCATTCATTAAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTAATGAATGAAGTGGTTCCTCGAGGAACCACTTCATTCATTAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTAATGAATGAAGTGGTTCCTCGAGGAACCACTTCATTCATTAAGA-3'

Other clones with same target sequence:

(none)