Construct: shRNA TRCN0000419729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015339.2-3880s21c1
DNA Barcode:
TAAGCTGGTGACTCATGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADNP (23394)
Gene Description:
activity dependent neuroprotector homeobox
Transcript:
RefSeq NM_015339.2 (NON-CURRENT)
Match location:
Position 3880 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGCTGGTGACTCATGAATA
Hairpin Sequence:
5'-CCGG-TAAGCTGGTGACTCATGAATA-CTCGAG-TATTCATGAGTCACCAGCTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGCTGGTGACTCATGAATACTCGAGTATTCATGAGTCACCAGCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGCTGGTGACTCATGAATACTCGAGTATTCATGAGTCACCAGCTTA-3'

Other clones with same target sequence:

(none)